Rapid Staining of Proteins in poluacrylamide gel
No more gradient gels!
electrophoresis buffer to AllView PAGE Buffer, and
get wide separation and 13 minutes
AllView PAGE Buffer is suitable for Leammli gels.
*When using precast gel, the applied gel concentration may be different.
Conventional SDS-PAGE buffer vs
AllView PAGE Buffer
Using a self-made gel with Tris- HCl buffer
(Laemmli method), the conventional SDS-PAGE
electrophoresis buffer (left, Tris-Glycine-SDS:
Laemmli method) and AllView PAGE Buffer (right)
At 6% gel concentration, conventional buffers
could not separate proteins below 45 kDa, but
AllView PAGE Buffer allowed a wide range of
separations, from 8 to 230 kDa, as in a gradient
Sample: DynaMarker Protein
MultiColor Stable II (code# DM660)
No need for expensive gradient gels
Electrophoresis completed in 13 minutes (mini
gel size, 250V)
Conventional analysis such as CBB staining,
silver staining, western blotting is possible.
20 x soluion
*The gel self-made by
Laemmli method is recommended. When using
precast gel, the applied gel concentration may
Comparison with various gel and electrophoresis