DynaMarker®Prestained Protein Molecular Weight Makers


		DynaExpress Rapid Staining of Proteins in poluacrylamide gel

AllView PAGE Buffer

No more gradient gels!

Change the electrophoresis buffer to AllView PAGE Buffer, and get wide separation and 13 minutes electrophoresis!

AllView PAGE Buffer is suitable for Leammli gels.
*When using precast gel, the applied gel concentration may be different.

Conventional SDS-PAGE buffer vs AllView PAGE Buffer
Using a self-made gel with Tris- HCl buffer (Laemmli method), the conventional SDS-PAGE electrophoresis buffer (left, Tris-Glycine-SDS: Laemmli method) and AllView PAGE Buffer (right) were compared.
At 6% gel concentration, conventional buffers could not separate proteins below 45 kDa, but AllView PAGE Buffer allowed a wide range of separations, from 8 to 230 kDa, as in a gradient gel.
Sample: DynaMarker Protein MultiColor Stable II (code# DM660)

Features

No need for expensive gradient gels　
Electrophoresis completed in 13 minutes (mini gel size, 250V)
Conventional analysis such as CBB staining, silver staining, western blotting is possible.
20 x soluion

*The gel self-made by Laemmli method is recommended. When using precast gel, the applied gel concentration may be different.

Comparison with various gel and electrophoresis buffers;

Running Buffer	Conventional SDS-PAGE electrophoresis buffer (Tris-Glycine-SDS: Laemmli method)			AllView PAGE Buffer
Gel type	Self-made gel (Tris-HCl buffer)		Gradient gel	Self-made gel (Tris-HCl buffer)
Gel concentration	6 %	15 %	4-20 %	6 %
Advantage	Large proteins	Small proteins	From small to large proteins	- From small to large proteins - Gradient gel is not needed
Disadvantage	Small proteins cannot be separated	Large proteins cannot be separated	Expensive	- From small to large proteins - Gradient gel is not needed
General electropholesis time (mini gel)	40 - 60 minutes at 200V	40 - 60 minutes at 200V	30 - 60 munutes at 200V	13 minutes at 250V
Images

Code	Product name	size	Product information
DS520	AllView PAGE Buffer	500 mL (20 x solution)

DynaExpress Rapid Staining of Proteins in poluacrylamide gel

AllView PAGE Buffer

DynaExpress
Rapid Staining of Proteins in poluacrylamide gel