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RNA Size Marker
Northern hybridization
Detection of RNA Sizemarkers on Hybridization
DNA fragments for DynaMarker® RNA High Probe
DNA fragments for DynaMarker® RNA High Probe is a product for detection of DynaMarker® RNA High (DM160) and DynaMarker® RNA High AGN (in DynaMarker® RNA Easy Measurement N, DM170) on hybridization.
Detects all RMA bands of DynaMarker® RNA High (DM160) and DynaMarker® RNA High AGN (in DynaMarker® RNA Easy Measurement N, DM170).
Preferable to generate isotopic end labeled probes and non-isotopic labeled probes.
Consists of 5' phosphorylated dsDNA fragments (5'-protruding ends) between 170 - 200 bp.
Profile of hybridization
Northen hybridization with DNA fragments for DynaMarker® RNA High Probe
DynaMarker® RNA High was electrophoresed in formaldehyde-agarose (1%) gel and transferred onto nylon membrane. DNA fragments for DynaMarker® RNA High Probe was labeled by non-isotopic method and hybridized to the nylon membrane.
After washing the blot, it was reacted with chemiluminescence substrate. Signal was exposed to a high speed film.
